HAMMOND ET. AL 2018 STEVENS LAB MCCARROLL LAB

Single cell RNA sequencing of microglia throughout the mouse lifespan and in the injured brain reveals complex cell-state changes

Microglia are the resident immune cells of the brain and are highly dynamic cells that respond to changes in their environment. We sequenced mouse microglia from E14.5 to 18 months as well as following demyelinating injury to capture the most comprehensive atlas of microglia cell state diversity to date. Use this searchable database to explore these state changes and gene expression changes in over 76,000 sequenced microglia from 41 mice. Download cluster-specific markers here.

Methods

It was essential to isolate microglia without activating them ex vivo. We developed a novel protocol to isolate microglia at 4 degrees to minimize any potential artifacts (see diagram). Detailed description of methods in published manuscript.

Manuscript

Hammond TR, Dufort C, Dissing-Olesen L, Giera S, Young A, Wysoker A, Walker A, Segel M, Saunders A, Macosko E, Marsh SM, Nemesh J, Ginhoux F, Chen J, Franklin RJ, Piao X, McCarroll S, and Stevens B. Single cell RNA sequencing of microglia throughout the mouse lifespan and in the injured brain reveals complex cell-state changes. Immunity (2018).
Link to paper